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This document serves as a general guide on how to approach and successfully calibrate a Picarro cavity ring-down spectrometer (CRDS). The steps described herein can be used on isotopic and/or concentration analyzers that measure CO2, CO, CH4, H2O, C2H2, C2H4, C2H6, CH2O, O2 and N2O. Additional analyzers may require special or extra steps to calibrate properly. If you are unsure if this document applies to your analyzer, please consult with Picarro support (support@picarro.com), your Picarro sales representative and Application Scientist.